Ukuqina kwezentengiso kokudla okusemathinini kubhekisele esimweni esiyinyumba esilinganiselwe lapho kungekho ama-microgenic microorganisms nama-microgenaganisms angezona azokwenziwa kabusha ngemuva kokuthola impilo ekheniwe ukuze uqinisekise ukuphepha kwesheya nekhwalithi. Ukudla okuthengiswayo kokudla okusemathinini ekuhlolweni kwe-microbiological kubonakaliswa yi-Rightility ehlobene, nama-microgenic microoorganisms, futhi akukho microgaganisms, futhi akukho microorganisms enganda kufinyelela kumathini egumbini lokushisa.
Ukuze uzuze amazinga wokudla okwamukelekayo kwezentengiso, inqubo yokukhiqiza yokudla ekheniwe ifaka izinqubo ezinjengezinto ezibonakalayo ezingavuthiwe, ukuwafaka, ukubekwa uphawu, ukufakwa kwensimbi, nokupakisha okufanele. Abakhiqizi abanobuchwepheshe obuthuthukile bokukhiqiza kanye nezidingo zokulawula ezisezingeni eliphakeme zinezinqubo eziyinkimbinkimbi futhi eziphelele zokukhiqiza.
Ubuchwepheshe bokuhlola ubuntu bezentengiselwano obuhleliwe ekuhlolweni kwe-microbiobiologiogiougigiologiogiougigiologiogiougigioliologioliologioliological buhlelekile, futhi ukuhlaziywa kwenqubo yalo ethile kuyakusebenzisa kangcono lobu buchwepheshe ekusebenzeni okusebenzayo kokudla okusemathinini. Inqubo ethile yokuhlolwa kwezentengiso okwenziwa ngezentengiselwano ekuhlolweni kwe-microbiobiological kungokulandelayo (ezinye izinhlaka zokuhlola ezivela eceleni ezinamandla zingaba nezinto eziningi zokuhlola):
1
Isiko le-bacterial elihlangene kungenye yezinqubo ezibalulekile ekuhlolweni kwezentengiso kokudla okusemathinini. Ngokwezomnotho ngokweqiniso okuqukethwe kwamasampula ahlaziyiwe, kanye nokuhlola kanye nokuhlola amakoloni ama-bacterial amasiko, izingxenye ze-microbial ekudleni okusemathinini zingahlolwa.
Ama-microgenic ama-microoorganisms ajwayelekile kumathini afaka phakathi kepha awagcini kumagciwane e-thermophilic, anjenge-bacillus stearthermophilus, ama-bacillus coabulans, ama-borppidium saccharolyticus, i-borturium niger, njll.; Amagciwane e-Mesophilic Anaerobic, njenge-Botulinum Toxin Closeridium, i-Couppidium Spotage, I-Closeridium Buttricianum, i-Closerdium Pasteuriamum, njll; Amagciwane e-Mesophilic aerobic, afana ne-bacillus subtilis, i-bacillus cereus, njll; Amagciwane angenayo akhiqiza anjenge-Escherhia Coli, i-streptococcus, imvubelo kanye nokubumba, isikhunta esilwa nokushisa nokunye. Ngaphambi kokwenza isiko lamagciwane ekhethini, qiniseka ukukala i-PH ye-Can ukuze ukhethe okuphakathi.
2. Isampula yezinto zokuhlola
Indlela yesampula ivame ukusetshenziselwa isampula yezinto zokuhlola zokudla okusemathinini. Lapho uvivinya ama-batches amakhulu wokudla okusemathinini, i-sampling ngokuvamile yenziwa ngokuya ngezinto ezifana nomenzi, uphawu lwentengiso, ukuhlukahluka kokudla okusemathinini noma isikhathi sokukhiqizwa. Ngamathini angajwayelekile njengamathini aceliwe, amathini aqhelelewe, ama-dents, kanye nokuvuvukala ekusakazweni kwabathengisi kanye nama-warehouse, i-sampling ethile ngokuvamile yenziwa ngokuya ngesimo. Kuyisidingo esiyisisekelo sokwesampula kwezinto zokuhlola ukukhetha indlela efanelekile yesampula ngokuya ngesimo sangempela, ukuze uthole izinto zokuhlola ezibonisa ikhwalithi yokudla okusemathinini.
3. Isampula yokugcina
Ngaphambi kokugcinwa kwesampula, imisebenzi efana nokulinganisa, ukugcina ukufudumala, futhi kuvulwa amathini okuvulwa. Sika isisindo se-net of the ngokuhlukile, kuya ngohlobo lwe-can, ludinga ukulumba ku-1G noma 2G. Kuhlanganiswe ne-PH kanye lokushisa, amathini agcinwa emazingeni okushisa ahlala njalo ezinsukwini eziyi-10; Amathini anamafutha noma avuthiwe ngesikhathi senqubo kufanele akhethwe ngokushesha ukuze ahlolwe. Ngemuva kokuthi inqubo yokulondolozwa kokushisa isiphelile, beka ongaphansi kwekamelo lokuvulwa kwe-aseptic. Ngemuva kokuvula i-can, sebenzisa amathuluzi afanele ukuthatha i-10-20 mg yokuqukethwe kusengaphambili ngesimo esiyinyumba, uyidlulisele esitsheni esilimele, bese uyigcina esiqandisini.
4.Isiko lokudla eliphansi le-acid
Ukutshala kokudla okunama-acid aphansi kudinga izindlela ezikhethekile: Ukutshala komhluzi onsomi onsomi ngo-36 ° C, ukutshalwa komhluzi onsomi onsomi ngo-55 ° C, kanye nokutshala inyama ephekiwe Medium ku-36 ° C. Imiphumela ifakwe futhi ichazwe, futhi ukuhlolwa okunembile okunembile kuhlelwe ngemuva kokuhlolwa kwemicroscopic, ukuze kuqinisekiswe ukunemba kwenhloso yezinhlobo zezinto ezibonakalayo ze-bacterial actification ukuhlonza kokudla okune-acid. Lapho amasiko ephakathi nendawo, gxila ekubhekeni ukukhiqizwa kwe-acid kanye nokukhiqizwa kwegesi kwamakoloni ama-microbial on the medium, kanye nokubukeka kanye nombala wamakoloni, ukuze uqinisekise izinhlobo ezithile ze-microbial ekudleni.
5. Ukuhlolwa Kwe-Microscopic
Ukuhlolwa kwe-smear ye-Microscopic kuyindlela esetshenziswa kakhulu yokuhlola okusetshenziswayo kokuhlolwa kwezimali okuthengiswayo, okudinga abahloli bekhwalithi abanolwazi ukuthi bagcwalise. Emvelweni oyinyumba, usebenzisa ukusebenza kwe-aseptic, faka uketshezi lwamagciwane ama-microorganisms aqukethe amasampula ahlaziyi asolwe emazingeni aphansi aphakathi nendawo, futhi abheke ukubonakala kwamagciwane ngaphansi kwesibonakhulu esiphakeme, ukuze anqume izinhlobo zama-microsorganisms ku-bacteroganisms. Ukuhlola, bese uhlela isinyathelo esilandelayo sesiko elicwengekileyo nokukhonjwa ukuze uqinisekise uhlobo lwamagciwane aqukethe. Lesi sinyathelo sidinga ikhwalithi ephezulu kakhulu yabahloli, futhi futhi sibe yisixhumanisi esingahlola kangcono ulwazi kanye namakhono ochwepheshe wabahloli.
I-6
Ngokudla okune-acid ngenani le-PH elingaphansi kuka-4,6, ukuhlolwa kwe-bacterium ubuthi ngokuvamile akusadingeki. Enqubweni yokutshala okukhethekile, ngaphezu kokusebenzisa impahla ye-acidic broth njenge-medium, kuyadingeka futhi ukusebenzisa umhluzi we-malt ekhipha umhluzi njengendlela yokutshala. Ngokufaka futhi ukuhlolwa kwemicroscopic kwamakoloni amagciwane amasiko, izinhlobo zamagciwane kuma-acid amathini zinganqunywa, ukuze zenzele inhloso eyengeziwe nokuhlolwa kwangempela kokuphepha kokudla kwamathini we-acid.
Isikhathi sePosi: Aug-10-2022